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Research and Clinical Interests

We characterized and cloned the cDNA of hNTH1, the human homolog of endonuclease III, a DNA N-glycosylase which initiates repair of oxidized pyrimidines in DNA. We noted a marked increase of spontaneously arising tumors mating mice lacking mNTH1 with ones lacking another repair enzyme mNEIL1, esp. the formation of hepatocellular carcinomas and uterine leiomyosarcomas. This demonstrates that spontaneous formation of oxidative damage to pyrimidines in genomic DNA, if not repaired, is tumorigenic.

Selected Publications

Ocampo-Hafalla MT, Altamirano A, Basu AK, Chan MK, Ocampo JE, Cummings A Jr, Boorstein RJ, Cunningham RP, Teebor GW. Repair of thymine glycol by hNth1 and hNeil1 is modulated by base pairing and cis-trans epimerization. DNA repair (Amsterdam). 2006;5:444–50. Cited in Pubmed; PMID 16446124.

Braithwaite EK, Kedar PS, Lan L, Polosina YY, Asagoshi K, Poltoratsky VP, Horton JK, Miller H, Teebor GW, Yasui A, Wilson SH. DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair. J Biol Chem. 2005 Sep 9;280(36):31641–7. Cited in Pubmed; PMID 16002405.

Marenstein DR, Wilson DM 3rd, Teebor GW. Human AP endonuclease (APE1) demonstrates endonucleolytic activity against AP sites in single-stranded DNA. DNA Repair (Amst). 2004 May 4;3(5):527–33. Cited in Pubmed; PMID 15084314.

Marenstein DR, Chan MK, Altamirano A, Basu AK, Boorstein RJ, Cunningham RP, Teebor GW. Substrate specificity of human endonuclease III (hNTH1). Effect of human APE1 on hNTH1 activity. Journal of Biological Chemistry. 2003 Mar 14;278(11):9005–12. Cited in Pubmed; PMID 12519758.

Ulbert S, Cross M, Boorstein RJ, Teebor GW, Borst P. Expression of the human hSMUG1 in Trypanosoma Brucei causes DNA damage and interferes with J. Biosynthesis. Nucleic Acids Res. 2002 Sep 15;30(18):3919–26. Cited in Pubmed; PMID 12235375.

Boorstein RJ, Cummings A Jr, Marenstein DR, Chan MK, Ma Y, Neubert TA, BrownSM, Teebor GW. Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1. J Biol Chem. 2001 Nov 9;276(45):41991–7. Cited in Pubmed; PMID J Biol Chem. 2001 Nov 9;276(45):41991–7. PMID: 11526119.

Marenstein DR, Ocampo MT, Chan MK, Altamirano A, Basu AK, Boorstein RJ, Cunningham RP, Teebor GW. Stimulation of human endonuclease III by Y box-binding protein 1 (DNA-bindingprotein B). Interaction between a base excision repair enzyme and a transcription factor. J Biol Chem. 2001 Jun 15;276(24):21242–9. Cited in Pubmed; PMID 11287425.

Chheda AD, Teebor GW, Cunningham RP. Identification, characterization, and purification of DNA glycosylase/AP lyases by reductive crosslinking to 2'-deoxyribooligonucleotides containing specific base lesions. Methods. 2000 Oct;22(2):180–7. Cited in Pubmed; PMID 11020333.

Hilbert TP, Chaung W, Boorstein RJ, Cunningham RP, Teebor GW. Cloning and expression of the cDNA encoding the human homologue of the DNA repair enzyme, Escherichia coli endonuclease III. J Biol Chem. 1997 Mar 7;272(10):6733–40. Cited in Pubmed; PMID 9045706.

Hilbert TP, Boorstein RJ, Kung HC, Bolton PH, Xing D, Cunningham RP, Teebor GW. Purification of a mammalian homologue of Escherichia coli endonuclease III: identification of a bovine pyrimidine hydrate-thymine glycol DNAse/AP lyase by irreversible cross linking to a thymine glycol-containing oligoxynucleotide. Biochemistry. 1996 Feb 27;35(8):2505–11. Cited in Pubmed; PMID 8611553.

Zuo S, Boorstein RJ, Teebor GW. Oxidative damage to 5-methylcytosine in DNA. Nucleic Acids Res. 1995 Aug 25;23(16):3239–43. Cited in Pubmed; PMID 7667100.

Boorstein RJ, Chiu LN, Teebor GW. A mammalian cell line deficient in activity of the DNA repair enzyme 5-hydroxymethyluracil-DNA glycosylase is resistant to the toxic effects of the thymidine analog 5-hydroxymethyl-2'-deoxyuridine. Cell Biol. 1992 Dec;12(12):5536–40. Cited in Pubmed; PMID 1448084.

Lustig MJ, Cadet J, Boorstein RJ, Teebor GW. Synthesis of the diastereomers of thymidine glycol, determination of concentrations and rates of interconversion of their cis-trans epimers at equilibrium and demonstration of differential alkali lability within DNA. Nucleic Acids Res. 1992 Sep 25;20(18):4839–45. Cited in Pubmed; PMID 1408799.

Selected Awards

Merit Award NIH 1988-1996

© 2008 NYU Department of Pathology