Research and Clinical Interests

Dr. Skok's research focuses on epigenetic regulation of V(D)J recombination. Her approach is to understand how nuclear organization of the antigen receptor genes affects their accessibility to the recombinase (RAG proteins). The aim is to understand how faithful DNA rearrangements are maintained in the context of the complex chromatin environment of developing B or T lymphocytes to prevent immune deficiency, autoimmunity and translocations involving Ig loci. Clinical interests include leukemia. Read more about research in the Skok Lab.

Selected Publications

Collins A, Hewitt SL, Chaumeil J, Sellars ML, Micsinai M, Allinne J, Parisi F, Nora E, Bolland DJ, Corcoran AE, Kluger Y, Bosselut R, Ellmeier W, Chong MMW, Littman DR, and Skok JA. RUNX Transcription Factor-Mediated Association of Cd4 and Cd8 Enables Coordinate Gene Regulation. Immunity. 2011 Mar 25;34(3):303-14. Cited in Pubmed; PMID 21435585. doi: 10.1016/j.immuni.2011.03.004. PMCID: PMC3101577.

Hewitt SL*, Yin B*, Ji Y, Chaumeil J, Marszalek K, Tenthorey J, Salvagiotto G, Steinel N, Ramsey LB, Ghysdael J, Farrar MA, Sleckman BP, Schatz DG, Busslinger M, Bassing CH*, Skok JA*. RAG1 and ATM coordinate monoallelic recombination and nuclear positioning of immunoglobulin loci. Nat Immunol. 2009 Jun;10(6):655-64. Epub 2009 May 17. doi: 10.1038/ni.1735. PMCID: PMC2693356.

Hewitt SL, Farmer D, Marszalek K, Cadera E, Liang HE, Xu Y, Schlissel MS, Skok JA. Association between the Igk and Igh immunoglobulin loci mediated by the 3' Igk enhancer induces 'decontraction' of the Igh locus. Nat Immunol. 2008 Apr;9(4):396-404. Epub 2008 Feb 24. Cited in Pubmed; PMID 18297074. doi: 10.1038/ni1567. PMCID: PMC2583163.

Johnson K, Hashimshony T, Sawai CM, Pongubala JM, Skok JA, Aifantis I, Singh H. Regulation of Immunoglobulin Light-Chain Recombination by the Transcription Factor IRF-4 and the Attenuation of Interleukin-7 Signaling. Immunity. 2008 Mar;28(3):335-45. Epub 2008 Feb 14. Cited in Pubmed; PMID 18280186. doi: 10.1016/j.immuni.2007.12.019.

Fitzsimmons S, Bernstein R, Skok JA, Max EE, Shapiro MA. Dynamic changes in accessibility, nuclear positioning, recombination, and transcription at the Ig kappa locus. J Immunol. 2007 Oct 15;179:5264-73. Cited in Pubmed; PMID 17911612.

Skok JA, Gisler R, Novatchkova M, Farmer D, de Laat W, Busslinger M. Reversible contraction by looping of the Tcra and Tcrb loci in rearranging thymocytes. Nat Immunol. 2007;8:378–87. Cited in Pubmed; PMID 17334367.

Roldan E, Fuxa M, Chong W, Martinez D, Novatchkova M, Busslinger M, Skok JA. Locus 'decontraction' and centromeric recruitment contribute to allelic exclusion of the immunoglobulin heavy-chain gene. Nat Immunol. 2005;6:31–41. Cited in Pubmed; PMID 15580273.

Goldmit M*, Ji Y*, Skok J*, Roldan E, Jung S, Cedar H, Bergman Y. Epigenetic ontogeny of the Igk locus during B cell development. Nat Immunol. 2005;6:198–203. Cited in Pubmed; PMID 15619624.

Fuxa M*, Skok J*, Souabni A, Salvagiotto G, Roldan E, Busslinger M. Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene. Genes Dev. 2004;18:411–22. Cited in Pubmed; PMID 15004008.

Kosak ST, Skok JA, Medina KL, Riblet R, Le Beau MM, Fisher AG, Singh H. Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. Science. 2002;296:158–62. Cited in Pubmed; PMID 11935030.

Skok JA, Brown KE, Azuara V, Caparros ML, Baxter J, Takacs K, Dillon N, Gray D, Perry RP, Merkenschlager M, et al. Nonequivalent nuclear location of immunoglobulin alleles in B lymphocytes. Nat Immunol. 2001;2:848–54. Cited in Pubmed; PMID 11526401.

Skok J, Poudrier J, Gray D. Dendritic cell-derived IL-12 promotes B cell induction of Th2 differentiation: a feedback regulation of Th1 development. J Immunol. 1999;163:4284–91. Cited in Pubmed; PMID 10510367.

Selected Reviews

Johnson K, Chaumeil J, Skok JA. Epigenetic regulation of V(D)J recombination. Essays Biochem. 2010 Sep 20;48(1):221-43. Cited in Pubmed; PMID 20822496. doi: 10.1042/bse0480221.

Hewitt SL, Chaumeil J, Skok JA. Chromosome dynamics and the regulation of V(D)J recombination. Immunol Rev. 2010 Sep;237(1):43-54. Cited in Pubmed; PMID 20727028. doi: 10.1111/j.1600-065X.2010.00931.x.

Organizational Associations

Associate member of the European Union Epigenome Network of Excellence

Selected Awards

2008 Whitehead Fellowship Award
2010 Recipient of a 5-Year Leukemia Lymphoma Society Scholar award for project entitled "Spatiotemporal regulation of Immunoglobulin recombination"

Additional Information

Research Profile