David W. O'Neill, M.D.
Assistant Professor of Pathology
Director, NYU Cancer Institute Vaccine and Cell Therapy Facility
Core Labs / Immunotherapy, Vaccine, Dendritic Cell, Immune Monitoring, Ikaros
oneild02@nyumc.org, (212) 263 8046 , (212) 263 8035
Fax: (212) 263 0270
400 East 34th Street, Rusk 718, New York, NY 10016
Research and Clinical Interests
Dr. O’Neill is interested in immunotherapies for cancer and chronic infections such as HIV/AIDS. He directs the NYU Cancer Institute’s Vaccine and Cell Therapy Facility, which provides the infrastructure and expertise to produce dendritic cell and other vaccines for human trials, and to monitor patients’ responses to therapy. The laboratory is currently focused on a study to test the immunogenicity of dendritic cells compared to incomplete Freund’s adjuvant in vaccines for malignant melanoma.
Selected Publications
Bogunovic D, O'Neill DW, Belitskaya-Levy I, Vacic V, Yu YL, Adams S, Darvishian F, Berman R, Shapiro R, Pavlick AC, Lonardi S, Zavadil J, Osman I, Bhardwaj N. Immune profile and mitotic index of metastatic melanoma lesions enhance clinical staging in predicting patient survival. Proc Natl Acad Sci U S A. 2009 Nov;Epub 2009 Nov 13. Cited in Pubmed; PMID 19915147. doi: 10.1073/pnas.0905139106.
Adams S, Lowes MA, O'Neill DW, Schachterle S, Romero P, Bhardwaj N. Lack of functionally active Melan-A(26-35)-specific T cells in the blood of HLA-A2+ vitiligo patients. J Invest Dermatol. 2008 Aug;128(8):1977-80. Epub 2008 Mar 13. Cited in Pubmed; PMID 18337837.
Adams S, O'Neill DW, Nonaka D, Hardin E, Chiriboga L, Siu K, Cruz CM, Angiulli A, Angiulli F, Ritter E, Holman RM, Shapiro RL, Berman RS, Berner N, Shao Y, Manches O, Pan L, Venhaus RR, Hoffman EW, Jungbluth A, Gnjatic S, Old L, Pavlick AC, Bhardwaj N. Immunization of malignant melanoma patients with full-length NY-ESO-1 protein using TLR7 agonist imiquimod as vaccine adjuvant. J Immunol. 2008 Jul 1;181(1):776-84. Cited in Pubmed; PMID 18566444.
Valmori D, Souleimanian NE, Tosello V, Bhardwaj N, Adams S, O'Neill D, Pavlick A, Escalon JB, Cruz CM, Angiulli A, Angiulli F, Mears G, Vogel SM, Pan L, Jungbluth AA, Hoffmann EW, Venhaus R, Ritter G, Old LJ, Ayyoub M. Vaccination with NY-ESO-1 protein and CpG in Montanide induces integrated antibody/Th1 responses and CD8 T cells through cross-priming. Proc Natl Acad Sci U S A. 2007 May 22;104(21):8947-52. Epub 2007 May 15. Cited in Pubmed; PMID 17517626.
Velazquez EF, Jungbluth AA, Yancovitz M, Gnjatic S, Adams S, O'Neill D, Zavilevich K, Albukh T, Christos P, Mazumdar M, Pavlick A, Polsky D, Shapiro R, Berman R, Spira J, Busam K, Osman I, Bhardwaj N. Expression of the cancer/testis antigen NY-ESO-1 in primary and metastatic malignant melanoma (MM)--correlation with prognostic factors. Cancer Immun. 2007;7:11-17. Cited in Pubmed; PMID 17625806.
O’Neill DW, Bhardwaj N. Armed and ready: How effector T cells deploy in reactive lymph nodes to modulate immunity. Nature Immunol. 2007;8(7):679-81. Cited in Pubmed; PMID 17579644.
Velazquez EF, Yancovitz M, Pavlick A, Berman R, Shapiro R, Bogunovic D, O'Neill D, Yu YL, Spira J, Christos PJ, Zhou XK, Mazumdar M, Nanus DM, Liebes L, Bhardwaj N, Polsky D, Osman I. Clinical relevance of Neutral Endopeptidase (NEP/CD10) in melanoma. J Transl Med. 2007;5:2-11. Cited in Pubmed; PMID 17207277.
O'Neill DW, Bhardwaj N. Exploiting dendritic cells for active immunotherapy of cancer and chronic infections. Mol Biotechnol. 2007;36:131-141. Cited in Pubmed; PMID 17914192.
O’Neill DW, Adams S, Bhardwaj N. Manipulating dendritic cell biology for the active immunotherapy of cancer. Blood. 2004;104:2235-46. Cited in Pubmed; PMID 15231572.
Lopez RA, Schoetz S, DeAngelis K, O'Neill D, Bank A. Multiple hematopoietic defects and delayed globin switching in Ikaros null mice. Proc Natl Acad Sci U S A. 2002;99:602-7. Cited in Pubmed; PMID 11805317.
O'Neill DW, Schoetz SS, Lopez RA, Castle M, Rabinowitz L, Shor E, Krawchuk D, Goll MG, Renz M, Seelig HP, Han S, Seong RH, Park SD, Agalioti T, Munshi N, Thanos D, Erdjument-Bromage H, Tempst P, Bank A. An Ikaros-containing chromatin-remodeling complex in adult-type erythroid cells. Mol Cell Biol. 2000;20:7572-82. Cited in Pubmed; PMID 11003653.
Xue H, O'Neill D, Wang X, Wolgemuth DJ, Bank A. HemT-3, an alternative transcript of mouse gene HemT specific to male germ cells. Gene. 1999;240:193-9. Cited in Pubmed; PMID 10564826.
Xue H, O'Neill D, Morrow J, Bank A. A novel mouse gene, HemT, encoding an hematopoietic cell-specific transcript. Gene. 1999;231(1-2):49-58. Cited in Pubmed; PMID 10231568.
O'Neill D, Yang J, Erdjument-Bromage H, Bornschlegel K, Tempst P, Bank A. Tissue-specific and developmental stage-specific DNA binding by a mammalian SWI/SNF complex associated with human fetal-to-adult globin gene switching. Proc Natl Acad Sci U S A. 1999;96:349-54. Cited in Pubmed; PMID 9892636.
Jackson, C. E., O’Neill, D. and Bank, A. Nuclear factor binding sites in human beta-globin IVS2. J Biol Chem. 1995;270:28448-56. Cited in Pubmed; PMID 7499351.
Selected Books
O’Neill D, Bhardwaj N. Generation of autologous peptide- and protein-pulsed dendritic cells for patient-specific immunotherapy. In: Ludewig B, Hoffmann MW, editors. Adoptive Immunotherapy – Methods and Protocols Methods in Molecular Medicine. Totowa, NJ: Humana Press; 2005. pp. 97-112.
O'Neill DW, Bhardwaj N. Differentiation of peripheral blood monocytes into dendritic cells. In: Curr Protoc Immunol. 2005. pp. Chapter 22:Unit 22F.4.
O’Neill DW, Bhardwaj N. Differentiation of peripheral blood monocytes into dendritic cells. In: Bierer BE, Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, editors. Current Protocols in Immunology. Hoboken, NJ: John Wiley & Sons; 2005. pp. 22F.4.1-22F.4.9.
Selected Awards
1988-1991 NIH National Research Service Award
1991-1993 Cooley's Anemia Foundation Fellowship
1993-1998 NIH Clinical Investigator Award
