Research and Clinical Interests

Mr. Lopez has been involved in the field of flow cytometry since 1977. His focus is to help facilitate the use of this technology to answer various biological questions. Mr. Lopez has contributed to a wide range of studies including the automated detection of malignant or premalignant cells, stem cell isolation, the study of T-cell receptor function, the detection and study of apoptotic cells, the study of effects of HIV infection in varied cell types.

Selected Publications

Mehandru S, Poles MA, Tenner-Racz K, Manuelli V, Jean-Pierre P, Lopez P, Shet A, Low A, Mohri H, Boden D, Racz P, Markowitz M. Mechanisms of gastrointestinal CD4+ T-cell depletion during acute and early human immunodeficiency virus type 1 infection. J Virol. 2007 Jan;81(2):599-612. Epub 2006 Oct 25. Cited in Pubmed; PMID 17065209.

Mehandru S, Poles MA, Tenner-Racz K, Jean-Pierre P, Manuelli V, Lopez P, Shet A, Low A, Mohri H, Boden D, Racz P, Markowitz M. Lack of Mucosal Immune Reconstitution during Prolonged Treatment of Acute and Early HIV-1 Infection. PLoS Med. 2006 Dec 5;3(12):e484. Cited in Pubmed; PMID 17147468.

Ho SH, Martin F, Higginbottom A, Partridge LJ, Parthasarathy V, Moseley GW, Lopez P, Cheng-Mayer C, Monk PN. Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J Virol. 2006 Jul;80(13):6487-96. Cited in Pubmed; PMID 16775336.

Brown A, Zhang H, Lopez P, Pardo CA, Gartner S. In vitro modeling of the HIV-macrophage reservoir. J Leukoc Biol. 2006;80:1127-35. Cited in Pubmed; PMID 16923921.

Roncarati R, Sestan N, Scheinfeld MH, Berechid BE, Lopez PA, Meucci O, McGlade JC, Rakic P, D'Adamio L. The gamma-secretase-generated intracellular domain of beta-amyloid precursor protein binds Numb and inhibits Notch signaling. Proc Natl Acad Sci U S A. 2002 May 14;99(10):7102-7. Cited in Pubmed; PMID 12011466.

Scheinfeld MH, Roncarati R, Vito P, Lopez PA, Abdallah M, D'Adamio L. Jun NH2-terminal kinase (JNK) interacting protein 1 (JIP1) binds the cytoplasmic domain of the Alzheimer's beta-amyloid precursor protein (APP). J Biol Chem. 2002 Feb 1;277(5):3767-75. Epub 2001 Nov 27. Cited in Pubmed; PMID 11724784.

Heagy W, Teng E, Lopez P, Finberg RW. Enkephalin receptors and receptor-mediated signal transduction in cultured human lymphocytes. Cell Immunol. 1999 Jan 10;191(10:34-48. Cited in Pubmed; PMID 9918685.

Schmid I, Nicholson JK, Giorgi JV, Janossy G, Kunkl A, Lopez PA, Perfetto S, Seamer LC, Dean PN. Biosafety guidelines for sorting of unfixed cells. Cytometry. 1997 Jun 1;28(2):99-117. Cited in Pubmed; PMID 9181299.

Castro JE, Listman JA, Jacobson BA, Wang Y, Lopez PA, Ju S, Finn PW, Perkins DL. Fas modulation of apoptosis during negative selection of thymocytes. Immunity. 1996 Dec;5(6):617-27. Cited in Pubmed; PMID 8986720.

Berardi AC, Wang A, Levine JD, Lopez P, Scadden DT. Functional isolation and characterization of human hematopoietic stem cells. Science. 1995 Jan 6;267(5194):104-8. Cited in Pubmed; PMID 7528940.

Hardin JA, Sherr DH, DeMaria M, Lopez PA. A simple fluorescence method for surface antigen phenotyping of lymphocytes undergoing DNA fragmentation. Immunol Methods. 1992 Sep 18;154(1):99-107. Cited in Pubmed; PMID 1401949.

Rodewald HR, Moingeon P, Lucich JL, Dosiou C, Lopez P, Reinherz EL. A population of early fetal thymocytes expressing Fc gamma RII/III contains precursors of T lymphocytes and natural killer cells. Cell. 1992 Apr 3;69(1):139-50. Cited in Pubmed; PMID 1532536.

Heagy W, Crumpacker C, Lopez PA, Finberg RW. Inhibition of immune functions by antiviral drugs. J Clin Invest. 1991 Jun;87(6):1916-24. Cited in Pubmed; PMID 1904068.

Chang HC, Moingeon P, Lopez P, Krasnow H, Stebbins C, Reinherz EL. Dissection of the human CD2 intracellular domain. Identification of a segment required for signal transduction and interleukin 2 production. J Exp Med. 1989 Jun 1;169(6):2073-83. Cited in Pubmed; PMID 2567337.

Wheeless LL, Patten SF, Berkan TK, Brooks CL, Gorman KM, Lesh SR, Lopez PA, Wood JC. Multidimensional slit-scan prescreening system: preliminary results of a single blind clinical study. Cytometry. 1984 Jan;5(1):1-8. Cited in Pubmed; PMID 6697819.

Selected Books

Lopez PA. High-speed sorting using the Cytomation MoFlo. In: Diamond RA, DeMaggio S, editors. In living color: protocols in flow cytometry and cell sorting. Berlin: Springer; 2000. pp. 577-84.

Adams PD, Lopez P, Sellers WR, Kaelin WG Jr. Fluorescence-activated cell sorting of transfected cells. In: Methods in Enzymology Volume 283. New York: Academic Press; 1997. pp. 59-72.

Selvaraj P, Low MG, Lopez P, Springer TA. Phosphatidylinositl-anchored antigens defined by non-lineage mAb. In: Knapp W et al, editor. Leucocyte typing IV: white cell differentation antigens. Oxford: Oxford University Press; 1989. pp. 734-44.

Organizational Associations

International Society for Analytical Cytometry; American Society for Clinical Pathology; MetroFlow