Research and Clinical Interests

We have 2 goals: 1) to devise an accurate, affordable diagnostic test for TB through work with promising antigens identified by immunological, proteomic and molecular approaches. 2) to understand how the inhaled M. tuberculosis bacilli adhere and enter the pulmonary cells, establish infection and disseminate. We have identified novel proteins expressed by the in vivo M. tuberculosis during the early asymptomatic stage of the infection; their role in the host-pathogen interaction is studied.

Selected Publications

Kinhikar A, Verma I, Chandra D, Singh KK, Weldingh K, Andersen P, Hsu T, Jacobs WR Jr, Laal S. Potential Role for ESAT-6 in Dissemination of M. tuberculosis via Human Lung Epithelial Cells. Mol Microbiol. 2010 Jan;75(1):92-106. Epub 2009 Nov 10. Cited in Pubmed; PMID 19906174. doi: 10.1111/j.1365-2958.2009.06959.x. PMCID: PMC2846543.

Singh KK, Sharma N, Vargas D, Liu Z, Belisle JT, Potharaju V, Wanchu A, Behera D, Laal S. Peptides of a Novel Mycobacterium tuberculosis-Specific Cell-wall Protein for Immunodiagnosis of Tuberculosis. J Infect Dis. 2009 Aug 15;200(4):571-81. Cited in Pubmed; PMID 19604115. doi: 10.1086/603539.

Steingart KR, Dendukuri N, Henry M, Schiller I, Nahid P, Hopewell PC, Ramsay A, Pai M, Laal S. Performance of Purified Antigens for Serodiagnosis of Pulmonary Tuberculosis: a Meta-analysis. Clin Vaccine Immunol. 2009 Feb;16(2):260-76. Epub 2008 Dec 3. Cited in Pubmed; PMID 19052159. doi: 10.1128/CVI.00355-08. PMCID: PMC2643545.

Shen G, Behera D, Bhalla M, Nadas A, Laal S. Peptide-based Antibody Detection for Tuberculosis Diagnosis. Clin Vaccine Immunol. 2009 Jan;16(1):49-54. Epub 2008 Nov 12. Cited in Pubmed; PMID 19005024. doi: 10.1128/CVI.00334-08. PMCID: PMC2620670.

Wanchu A, Dong Y, Sethi S, Myneedu VP, Nadas A, Liu Z, Belisle J, Laal S. Biomarkers for Clinical and Incipient Tuberculosis: Performance in a TB-endemic Country. PLoS One. 2008 Apr 30;3(4):e2071. Cited in Pubmed; PMID 18446210. doi: 10.1371/journal.pone.0002071. PMCID: PMC2323108.

Steingart KR, Henry M, Laal S, Hopewell PC, Ramsay A, Menzies R, Cunningham J, Weldingh K, Pai M. Commercial serological antibody detection tests for the diagnosis of pulmonary tuberculosis: a systematic review. PLoS Medicine. 2007;4:1-20. Cited in Pubmed; PMID 17564490.

Steingart KR, Henry M, Laal S, Hopewell PC, Ramsay A, Menzies R, Cunningham J, Wldingh K, Pai M. A systematic review of commercial serological antibody detection tests for the diagnosis of extrapulmonary tuberculosis. Thorax. 2007;62:911-918. Cited in Pubmed; PMID 17675320.

Sartain MJ, Slayden RA, Singh KK, Laal S, Belisle JT. Disease state differentiation and identification of tuberculosis biomarkers via native antigen profiling. Mol Cell Proteomics. 2006;5:2102-13. Cited in Pubmed; PMID 16899542.

Achkar JM, Dong Y, Holzman RS, Belisle J, Kourbeti IS, Sherpa T, Condos R, Rom W, Laal S. M.tuberculosis Malate Synthase/MPT51-based serodiagnostic assay as adjunct to rapid identification of pulmonary tuberculosis. Clin Vaccine Immunol. 2006;13:1291-3. Cited in Pubmed; PMID 17090645.

Kinhikar AG, Vargas D, Li H, Mahaffey SB, Hinds L, Belisle JT, Laal S. Mycobacterium tuberculosis malate synthase is a laminin binding adhesin. Mol Microbiol. 2006;64:999-1013. Cited in Pubmed; PMID 16677310.

Singh KK, Dong Y, Patibandla SA, McMurray DN, Arora VK, Laal S. Immunogenicity of Mycobacterium tuberculosis PPE55 (Rv3347c) protein during incipient and clinical Tuberculosis. Infect Immun. 2005;73:5004-14. Cited in Pubmed; PMID 16041015.

Singh KK, Dong Y, Belisle JT, Harder J, Arora VK, Laal S. Antigens of Mycobacterium tuberculosis recognized by antibodies during incipient, subclinical tuberculosis. Clin Diagn Lab Immunol. 2005;12(2):354-8. Cited in Pubmed; PMID 15699433.

Singh KK, Dong Y, Hinds L, Keen MA, Belisle JT, Zolla-Pazner S, Achkar JM, Nadas AJ, Arora VK, Laal S. Combined use of serum and urinary antibody for diagnosis of TB. J Infect Dis. 2003;188:371-7. Cited in Pubmed; PMID 12870118.

Samanich KM, Belisle JT, Laal S. Homogeneity of Antibody responses in Tuberculosis. Infect Immun. 2001;69:4600-9. Cited in Pubmed; PMID 11402004.

Singh KK, Zhang X, Chien P, Laal S. Antigens of M. tuberculosis expressed during early TB: Immmundominance of Repetitive Proteins. Infect Immun. 2001;69:4185-91. Cited in Pubmed; PMID 11349098.

Selected Books

Laal S. New Diagnostic Techniques: Clinical Relevance. In: Arora VK, editor. Practical Approach to Tuberculosis Management. New Delhi: Jaypee Brothers Medical Publishers; 2006. pp. 361-371.

Laal S, Skieky Y. Immune Based Methods of Diagnosis. In: Cole ST, Gicquel B, McMurray DN, Eisenach KD, Jacobs WR, editors. Tuberculosis and the Tubercle Bacillus. Second Edition. Washington DC: ASM Press; 2004. pp. 71-84.

Laal S. Immunodiagnosis. In: Rom WN, Garay SM, editors. Tuberculosis. Second Edition. New York: Lippincot Williams & Wilkins; 2003. pp. 185-192.

Laal S. Humoral Responses to M. tuberculosis. In: Rom, W.N. and S. M. Garay, editors. Tuberculosis. Boston: Little, Brown and Co; 1996. pp. 335.

Zolla-Pazner S, Laal S, Burda S, Buchbinder A. Human monoclonal antibodies to HIV-1 define synergistic activities leading to enhanced neutralization. In: Antibiotics and Chemotherapy. Advanced Technologies in Research, Diagnosis and Treatment of AIDS and Oncology. Basel: Karger; 1994. pp. 38.

Laal S, Zolla-Pazner S. Epitopes of HIV-1 glycoproteins recognized by the human immune response. In: Norrby E, editor. Chemical Immunology. Basel: Karger; 1993. pp. 91.

Nath I, Laal S. Immunofluorescence as a tool for investigation of granulomatous disease. In: Madhvan M, editor. Immunodiagnostic Procedures in Laboratory Medicine. Madras: Graphico Associates Press; 1987. pp. 80.

Organizational Associations

Consultant, Blood Products Advisory Committee, FDA; Member, UNICEF/UNDP/World Bank/WHO Special Program for a Systematic Review of TB; Member, Scientific Advisory Committee, Super Religare Labs, India; Member, WHO Specimen Bank Committee