Research and Clinical Interests

The Krogsgaard laboratory is using novel imaging and biophysical methods to offer new insights into the mechanism of how robust andfunctional T-cell responses can be generated against “self” (tumor) antigens. This is with the ultimate goal of targeting individual molecular components to improve vaccine development and therapies for cancer and other immune-mediated diseases.

Selected Publications

Corse E, Gottschalk RA, Krogsgaard M, Allison JP. Attenuated T cell responses to a high-potency ligand in vivo. PLoS Biol. 2010 Sep 14;8(9):e1000481. Cited in Pubmed; PMID 20856903. doi: 10.1371/journal.pbio.1000481. PMCID: PMC2939023.

Juang J, Ebert PJ, Feng D, Garcia KC, Krogsgaard M, Davis MM. Peptide-MHC heterodimers show that thymic positive selection requires a more restricted set of self-peptides than negative selection. J Exp Med. 2010 Jun 7;207(6):1223-34. Epub 2010 May 10. Cited in Pubmed; PMID 20457759. doi: 10.1084/jem.20092170. PMCID: PMC2882826.

Call MJ, Xing X, Cuny GD, Seth NP, Altmann DM, Fugger L, Krogsgaard M, Stein RL, Wucherpfennig KW. In vivo enhancement of peptide display by MHC class II molecules with small molecule catalysts of peptide exchange. J Immunol. 2009 May 15;182(10):6342-52. Cited in Pubmed; PMID 19414787. doi: 10.4049/jimmunol.0803464. PMCID: PMC2695398.

Qi S, Krogsgaard M, Davis MM, Chakraborty AK. Molecular flexibility can influence the stimulatory ability of receptor-ligand interactions at cell-cell junctions. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4416-21. Epub 2006 Mar 13. Cited in Pubmed; PMID 16537380.

Maynard J, Adams EJ, Krogsgaard M, Petersson K, Liu CW, Garcia KC. High-level bacterial secretion of single-chain alphabeta T-cell receptors. J Immunol Methods. 2005 Nov 30;306(1-2):51-67. Epub 2005 Sep 12. Cited in Pubmed; PMID 16198365.

Krogsgaard M, Li QJ, Sumen C, Huppa JB, Huse M, Davis MM. Agonist/endogenous peptide-MHC heterodimers drive T cell activation and sensitivity. Nature. 2005 Mar 10;434(7030):238-43. Epub 2005 Feb 20. Cited in Pubmed; PMID 15724150.

Kasson PM, Huppa JB, Krogsgaard M, Davis MM, Brunger AT. Quantitative imaging of lymphocyte membrane protein reorganization and signaling. Biophys J. 2005 Jan;88(1):579-89. Epub 2004 Oct 22. Cited in Pubmed; PMID 15501943.

Ranheim EA, Tarbell KV, Krogsgaard M, Mallet-Designe V, Teyton L, McDevitt HO, Weissman IL. Selection of aberrant class II restricted CD8+ T cells in NOD mice expressing a glutamic acid decarboxylase (GAD)65-specific T cell receptor transgene. Autoimmunity. 2004 Dec;37(8):555-67. Cited in Pubmed; PMID 15763918.

Krogsgaard M, Prado N, Adams EJ, He XL, Chow DC, Wilson DB, Garcia KC, Davis MM. Evidence that structural rearrangements and/or flexibility during TCR binding can contribute to T cell activation. Mol Cell. 2003 Dec;12(6):1367-78. Cited in Pubmed; PMID 14690592.

Anikeeva N, Lebedeva T, Krogsgaard M, Tetin SY, Martinez-Hackert E, Kalams SA, Davis MM, Sykulev Y. Distinct molecular mechanisms account for the specificity of two different T-cell receptors. Biochemistry. 2003 Apr 29;42(16):4709-16. Cited in Pubmed; PMID 12705834.

Irvine DJ, Purbhoo MA, Krogsgaard M, Davis MM. Direct observation of ligand recognition by T cells. Nature. 2002 Oct 24;419(6909):845-9. Cited in Pubmed; PMID 12397360.

Krogsgaard M, Wucherpfennig KW, Cannella B, Hansen BE, Svejgaard A, Pyrdol J, Ditzel H, Raine C, Engberg J, Fugger L. Visualization of myelin basic protein (MBP) T cell epitopes in multiple sclerosis lesions using a monoclonal antibody specific for the human histocompatibility leukocyte antigen (HLA)-DR2-MBP 85-99 complex. J Exp Med. 2000 Apr 17;191(8):1395-412. Cited in Pubmed; PMID 10770805.

Madsen LS, Andersson EC, Jansson L, Krogsgaard M, Andersen CB, Engberg J, Strominger JL, Svejgaard A, Hjorth JP, Holmdahl R, Wucherpfennig KW, Fugger L. A humanized model for multiple sclerosis using HLA-DR2 and a human T-cell receptor. Nat Genet. 1999 Nov;23(3):343-7. Cited in Pubmed; PMID 10610182.

Selected Books and Reviews

Krogsgaard M, Juang J, Davis MM. A role for "self" in T-cell activation. Semin Immunol. 2007 Aug;19(4):236-44. Epub 2007 Jun 4. Cited in Pubmed; PMID 17548210.

Davis MM, Krogsgaard M, Huse M, Huppa J, Lillemeier BF, Li QJ. T cells as a self-referential, sensory organ. Annu Rev Immunol. 2007;25:681-95. Cited in Pubmed; PMID 17291190. doi: 10.1146/annurev.immunol.24.021605.090600.

Krogsgaard M, Davis MM. How T cells 'see' antigen. Nat Immunol. 2005 Mar;6(3):239-43. Cited in Pubmed; PMID 15716973.

Krogsgaard M, Huppa JB, Purbhoo MA, Davis MM. Linking molecular and cellular events in T-cell activation and synapse formation. Semin Immunol. 2003 Dec;15(6):307-15. Cited in Pubmed; PMID 15001169.

Davis MM, Krogsgaard M, Huppa JB, Sumen C, Purbhoo MA, Irvine DJ, Wu LC, Ehrlich L. Dynamics of cell surface molecules during T cell recognition. Annu Rev Biochem. 2003;72:717-42. Cited in Pubmed; PMID 14527326. doi: 10.1146/annurev.biochem.72.121801.161625.

Engberg J, Johansen LK, Westengaard-Hildinge M, Riise ES, Albrechtsen B. Phage-display libraries of murine and human anitbody fab fragments. In: Rapley R, editor. The Nucleic acid protocols handbook. Totowa, N.J.: Humana Press; 2000. pp. 449-478.

Engberg J, Krogsgaard M, Fugger L. Recombinant antibodies with the antigen-specific, MHC restricted specificity of T cells: novel reagents for basic and clinical investigations and immunotherapy. Immunotechnology. 1999 Mar;4(3-4):273-8. Cited in Pubmed; PMID 10231096.

Organizational Associations

The American Association of Immunologists (AAI); The American Association for the Advancement of Science (AAAS); American Chemical Society (ACS)

Selected Awards

1999-2002 The Alfred Benzon Foundation, Denmark, Research Fellowship
1999 The Danish Pasteur Society, Travel Award
1999 The Plasmid Foundation, Travel Award
1999 The Danish University of Pharmaceutical Sciences, Travel Award
2003-2004 The Danish Medical Research Council, Denmark, Research Fellowship
2007 The Kimmel Scholar Award - declined
2007 Pew Scholar Award
2007 Cancer Research Institute (CRI) Investigator Award
2008-2012 NIH EUREKA (Exceptional Unconventional Research Enabling Knowledge Advancement) Award recipient
2009-2012 American Cancer Society Research Scholar

Additional Information

Detailed CV: Krogsgaard_2010_CV.pdf