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Research and Clinical Interests

Dr. Klein is interested in the regulation of DNA repair and recombination pathways that act at damaged replication forks to prevent genomic instability. She currently focuses on DNA helicases and SWI/SNF proteins that regulate the action of the recombinase Rad51 in directing the repair of DNA lesions. Other interests include the role of cohesins in DNA repair, the types of genomic instability that occur in DNA damage checkpoint deficient mutants and novel DNA repair factors for cell lifespan.

Selected Publications

Sung P, Klein H. Mechanism of homologous recombination: mediators and helicases take on regulatory functions. Nat Rev Mol Cell Biol. 2007 Oct;7(10):739-50. Cited in Pubmed; PMID 16926856.

Kwon Y, Chi P, Roh DH, Klein H, Sung P. Synergistic action of the Saccharomyces cerevisiae homologous recombination factors Rad54 and Rad51 in chromatin remodeling. DNA Repair(Amst). 2006 Oct 1;6(10):1496-506. Cited in Pubmed; PMID 17544928.

Chi P, Kwon Y, Seong C, Epshtein A, Lam I, Sung P, Klein HL. Yeast recombination factor Rdh54 functionally interacts with the Rad51 recombinase and catalyzes Rad51 removal from DNA. J Biol Chem. 2006 Sep 8;281(36):26268(79). Cited in Pubmed; PMID 16831867.

Monroe DS Jr, Leitzel AK, Klein HL, Matson SW. Biochemical and genetic characterization of Hmi1p, a yeast DNA helicase involved in the maintenance of mitochondrial DNA. Yeast. 2005 Dec;22(16):1269-86. Cited in Pubmed; PMID 16358299.

Xu H, Boone C, Klein HL. Mrc1 is required for sister chromatid cohesion to aid in recombination repair of spontaneous damage. Mol Cell Biol. 2004 Aug;24(16):7082-90. Cited in Pubmed; PMID 15282308.

Smirnova M, Van Komen S, Sung P, Klein HL. Effects of tumor-associated mutations on Rad54 functions. J Biol Chem. 2004 Jun 4;279(23):24081-8. Cited in Pubmed; PMID 15056673.

Krejci L, Macris M, Li Y, Van Komen S, Villemain J, Ellenberger T, Klein H, Sung P. Role of ATP hydrolysis in the antirecombinase function of Saccharomyces cerevisiae Srs2 protein. J Biol Chem. 2004 May 28;279(22):23193-9. Cited in Pubmed; PMID 15047689.

Smirnova M, Klein HL. Role of the error-free damage bypass postreplication repair pathway in the maintenance of genomic stability. Mutat Res. 2003 Nov 27;532(1-5):117-25. Cited in Pubmed; PMID 14643433.

Van Komen S, Reddy MS, Krejci L, Klein H, Sung P. ATPase and DNA helicase activities of the Saccharomyces cerevisiae anti-recombinase Srs2. J Biol Chem. 2003 Nov 7;278(45):44331-7. Cited in Pubmed; PMID 12966095.

Krejci L, Van Komen S, Li Y, Villemain J, Reddy MS, Klein H, Ellenberger T, Sung P. DNA helicase Srs2 disrupts the Rad51 presynaptic filament. Nature. 2003 May 15;423(6937):305-9. Cited in Pubmed; PMID 12748644.

Merker RJ, Klein HL. Role of transcription in plasmid maintenance in the hpr1Delta mutant of Saccharomyces cerevisiae. Mol Cell Biol. 2002 Dec;22(24):8763-73. Cited in Pubmed; PMID 12446793.

Selected Books

Klein HL. DNA helicases in recombination. In: Rothstein R, Aguilera A, editors. Topics in Current Genetics: Molecular Genetics of Recombination. Vol. 17. Heidelberg: Springer-Verlag; 2007. pp. 135-168.

Klein HL. Recombination and Genome Rearrangements. In: Meyers RA, editor. Encyclopedia of Molecular Cell Biology and Molecular Medicine. Vol. 12. Weinheim: Wiley-VCH Publishers; 2005. pp. 1-22.

Organizational Associations

Genetics Society of America; Editorial Boards, Nucleic Acids Research, DNA Repair

© 2008 NYU Department of Pathology