Research and Clinical Interests

Dr. Gorny is studying the humoral immune response against HIV-1 by generating human monoclonal antibodies (mAbs) that neutralize HIV-1 primary isolates. These mAbs are used to characterize viral antigens that may be used for vaccine development. He is also investigating immunoglobulin gene usage for anti-HIV-1 human mAbs and analyzing the correlation between preferentially used genes and mAb function, particularly with respect to their neutralizing activity.

Selected Publications

Burke V, Williams C, Sukumaran M, Kim SS, Li H, Wang XH, Gorny MK, Zolla-Pazner S, Kong XP. Structural basis of the cross-reactivity of genetically related human anti-HIV-1 mAbs: implications for design of V3-based immunogens. Structure. 2009 Nov;17(11):1538-46. Cited in Pubmed; PMID 19913488. doi: 10.1016/j.str.2009.09.012.

Gorny MK, Wang XH, Williams C, Volsky B, Revesz K, Witover B, Burda S, Urbanski M, Nyambi P, Krachmarov C, Pinter A, Zolla-Pazner S, Nadas A. Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1. Mol Immunol. 2009 Feb;46(5):917-26. Cited in Pubmed; PMID 18952295. doi: 10.1016/j.molimm.2008.09.005. PMCID: PMC2693011.

Kimura T, Wang XH, Williams C, Zolla-Pazner S, Gorny MK. Human monoclonal antibody 2909 binds to pseudovirions expressing trimers but not monomeric HIV-1 envelope proteins. Hum Antibodies. 2009;18(1-2):35-40. Cited in Pubmed; PMID 19478397.

Yuan W, Li X, Kasterka M, Gorny MK, Zolla-Pazner S, Sodroski J. Oligomer-specific conformations of the human immunodeficiency virus (HIV-1) gp41 envelope glycoprotein ectodomain recognized by human monoclonal antibodies. AIDS Res Hum Retroviruses. 2009;25(3):319-28. Cited in Pubmed; PMID 19292593. doi: 10.1089/aid.2008.0213.

Visciano ML, Tuen M, Gorny M, Hioe CE. In vivo alteration of humoral responses to HIV envelope glycoprotein gp120 by antibodies to the CD4-binding site of gp120. Virology. 2008 Mar 15;372(2):409-20. Epub 2007 Dec 4. Cited in Pubmed; PMID 18054978. doi: 10.1016/j.virol.2007.10.044 . PMCID: PMC2288784.

Dhillon AK, Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA. Structure determination of an anti-HIV-1 Fab 447-52D-peptide complex from an epitaxially twinned data set. Acta Crystallogr D Biol Crystallogr. 2008;D64:792-802. Cited in Pubmed; PMID 18566514. PMCID: PMC2631122.

Dadachova E, Patel MC, Toussi S, Apostolidis C, Morgenstern A, Brechbiel MW, Gorny MK, Zolla-Pazner S, Casadevall A, Goldstein H. Targeted killing of virally infected cells by radiolabeled antibodies to viral proteins. PLoS Med. 2006;3(11):e427. Cited in Pubmed; PMID 17090209. doi: 10.1371/journal.pmed.0030427. PMCID: PMC1630718.

Gorny MK, Williams C, Volsky B, Revesz K, Wang XH, Burda S, Kimura T, Konings FA, Nadas A, Anyangwe CA, Nyambi P, Krachmarov C, Pinter A, Zolla-Pazner S. Cross-clade neutralizing activity of human anti-V3 monoclonal antibodies derived from the cells of individuals infected with non-B clades of human immunodeficiency virus type 1. J Virol. 2006;80(14):6865-72. Cited in Pubmed; PMID 16809292.

Gorny MK, Stamatatos L, Volsky B, Revesz K, Williams C, Wang XH, Cohen S, Staudinger R, Zolla-Pazner S. Identification of a new quaternary neutralizing epitope on human immunodeficiency virus type 1 virus particles. J Virol. 2005;79(8):5232-7.

Gorny MK, Revesz K, Williams C, Volsky B, Louder MK, Anyangwe CA, Krachmarov C, Kayman SC, Pinter A, Nadas A, Nyambi PN, Mascola JR, Zolla-Pazner S. The V3 loop is accessible on the surface of most human immunodeficiency virus type 1 primary isolates and serves as a neutralization epitope. J Virol. 2004;78(5):2394-404. Cited in Pubmed; PMID 14963135.

Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA. Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D. Structure. 2004;12(2):193-204. Cited in Pubmed; PMID 14962380.

Zolla-Pazner S, Zhong P, Revesz K, Volsky B, Williams C, Nyambi P, Gorny MK. The cross-clade neutralizing activity of a human monoclonal antibody is determined by the GPGR V3 motif of HIV type 1. AIDS Res Hum Retroviruses. 2004;20(11):1254-8. Cited in Pubmed; PMID 15588347.

Gorny MK, Williams C, Volsky B, Revesz K, Cohen S, Polonis VR, Honnen WJ, Kayman SC, Krachmarov C, Pinter A, Zolla-Pazner S. Human monoclonal antibodies specific for conformation-sensitive epitopes of V3 neutralize human immunodeficiency virus type 1 primary isolates from various clades. J Virol. 2002;76(18):9035-45. Cited in Pubmed; PMID 12186887.

Gorny MK, Zolla-Pazner S. Recognition by human monoclonal antibodies of free and complexed peptides representing the prefusogenic and fusogenic forms of human immunodeficiency virus type 1 gp41. J Virol. 2000;74(13):6186-92. Cited in Pubmed; PMID 10846104.

Gorny MK, VanCott TC, Williams C, Revesz K, Zolla-Pazner S. Effects of oligomerization on the epitopes of the human immunodeficiency virus type 1 envelope glycoproteins. Virology. 2000;267(2):220-8. Cited in Pubmed; PMID 10662617.

Selected Books

Gorny MK, Zolla-Pazner S. Human monoclonal antibodies that neutralize HIV-1. In: Korber BTM, Brander C, Haynes BF, Koup R, Moore JP, Walker BD, Watkins DI, editors. HIV Immunology and HIV/SIV Vaccine Databases 2003. Los Alamos, N.M.: Los Alamos National Laboratory, Theoretical Biology and Biophysics; 2004. pp. 37-51.

Gorny MK. Production of human monoclonal antibodies via fusion of Epstein-Barr virus-transformed lymphocytes with heteromyeloma. In: Celis J, editor. Cell Biology: A Laboratory Handbook. San Diego: Academic Press; 1994. pp. 276-81.

Vitetta ES, Till MA, Ghetie V, Uhr JW, Patton JS, Gregory T, Patzer EJ, Porter JP, Capon DJ, Gorny MK, Zolla-Pazner S. Immunoconjugates containing ricin A chain can kill cell lines of HIV infected T cells and monocytes. In: Baldwin RW, Vyers VS, Mann RD, editors. Monoclonal Antibodies and Immunoconjugates. Park Ridge, NJ: Parthenon Publishing Group; 1990. pp. 79-94.

Gorny MK, Pinter A, Zolla-Pazner S. Specific immunity to HIV and other retroviral infections. In: Rotterdam H et al., editor. Progress in AIDS Pathology. New York: Field & Wood Publ.; 1989. pp. 181-199.

Organizational Associations

American Association of Immunologists; American Association for the Advancement of Science