Research and Clinical Interests

Adaptive immunity requires a network of cell-cell interactions that are orchestrated by chemokines, adhesion molecules and antigen receptors. We study the biology of T lymphocyte activation and differentiation using integrated in vitro and in vivo microscopic observation and mouse genetics. Specific contexts include vaccination, autoimmunity, asthma, bacterial and viral infection and cancer immunotherapy.

Selected Publications

Victora GD, Schwickert TA, Fooksman DR, Kamphorst AO, Meyer-Hermann M, Dustin ML, Nussenzweig MC. Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter. Cell. 2010 Nov 12;143(4):592-605. Cited in Pubmed; PMID 21074050. doi: 10.1016/j.cell.2010.10.032. PMCID: PMC3035939 [Available on 2011/11/12].

Vardhana S, Choudhuri K, Varma R, Dustin ML. Essential Role of Ubiquitin and TSG101 Protein in Formation and Function of the Central Supramolecular Activation Cluster. Immunity. 2010 Apr 23;32(4):531-40. Cited in Pubmed; PMID 20399684. doi: 10.1016/j.immuni.2010.04.005.

Zanin-Zhorov A, Ding Y, Kumari S, Attur M, Hippen KL, Brown M, Blazar BR, Abramson SB, Lafaille JJ, Dustin ML. Protein Kinase C-theta Mediates Negative Feedback on Regulatory T Cell Function. Science. 2010 Apr 16;328(5976):372-6. Epub 2010 Mar 25. Cited in Pubmed; PMID 20339032. doi: 10.1126/science.1186068. PMCID: PMC2905626.

Kim JV, Kang SS, Dustin ML, McGavern DB. Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis. Nature. 2009 Jan 8;457:191-5. Epub 2008 Nov 16. Cited in Pubmed; PMID 19011611. doi: 10.1038/nature07591.

Milstein O, Tseng SY, Starr T, Llodra J, Nans A, Liu M, Wild MK, van der Merwe PA, Stokes DL, Reisner Y, Dustin ML. Nanoscale Increases in CD2-CD48-mediated Intermembrane Spacing Decrease Adhesion and Reorganize the Immunological Synapse. J Biol Chem. 2008 Dec 5;283(49):34414-22. Epub 2008 Sep 30. Cited in Pubmed; PMID 18826951. doi: 10.1074/jbc.M804756200.

Kim N, Stiegler AL, Cameron TO, Hallock PT, Gomez AM, Huang JH, Hubbard SR, Dustin ML, Burden SJ. Lrp4 is a receptor for Agrin and forms a complex with MuSK. Cell. 2008 Oct 17;135(2):334-42. Epub 2008 Oct 9. Cited in Pubmed; PMID 18848351. doi: 10.1016/j.cell.2008.10.002.

Vasiliver-Shamis G, Tuen M, Wu TW, Starr T, Cameron TO, Thomson R, Kaur G, Liu J, Visciano ML, Li H, Kumar R, Ansari R, Han DP, Cho MW, Dustin ML, Hioe CE. Human immunodeficiency virus type 1 envelope gp120 induces a stop signal and virological synapse formation in noninfected CD4+ T cells. J Virol. 2008 Oct;82(19):9445-57. Epub 2008 Jul 16. Cited in Pubmed; PMID 18632854. doi: 10.1128/JVI.00835-08. PMCID: PMC2546991.

Matsumura S, Wang B, Kawashima N, Braunstein S, Badura M, Cameron TO, Babb JS, Schneider RJ, Formenti SC, Dustin ML, Demaria S. Radiation-induced CXCL16 release by breast cancer cells attracts effector T cells. J Immunol. 2008 Sep 1;181(5):3099-107. Cited in Pubmed; PMID 18713980. PMCID: PMC2587101.

Kirchhof MG, Chau LA, Lemke CD, Vardhana S, Darlington PJ, Márquez ME, Taylor R, Rizkalla K, Blanca I, Dustin ML, Madrenas J. Modulation of T cell activation by stomatin-like protein 2. J Immunol. 2008 Aug 1;181(3):1927-36. Cited in Pubmed; PMID 18641330.

Tolentino TP, Wu J, Zarnitsyna VI, Fang Y, Dustin ML, Zhu C. Measuring diffusion and binding kinetics by contact area FRAP. Biophys J. 2008 Jul;95(2):920-30. Epub 2008 Apr 4. Cited in Pubmed; PMID 18390627. doi: 10.1529/biophysj.107.114447. PMCID: PMC2440437.

Thauland TJ, Koguchi Y, Wetzel SA, Dustin ML, Parker DC. Th1 and Th2 cells form morphologically distinct immunological synapses. J Immunol. 2008 Jul 1;181(1):393-9. Cited in Pubmed; PMID 18566405. PMCID: PMC2441916.

Wu J, Fang Y, Zarnitsyna VI, Tolentino TP, Dustin ML, Zhu C. A coupled diffusion-kinetics model for analysis of contact-area FRAP experiment. Biophys J. 2008 Jul;95(2):910-9. Epub 2008 Apr 4. Cited in Pubmed; PMID 18390628. doi: 10.1529/biophysj.107.114439. PMCID: PMC2440458.

Shen K, Thomas VK, Dustin ML, Kam LC. Micropatterning of CD28 ligands enhances CD4+ T cell costimulation. Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7791-6. Epub 2008 May 27. Cited in Pubmed; PMID 18505845. doi: 10.1073/pnas.0710295105. PMCID: PMC2409411.

DeMond AL, Mossman KD, Starr T, Dustin ML, Groves J. T Cell Receptor microcluster transport through molecular mazes reveals mechanism of translocation. Biophys J. 2008 Apr 15;94(8):3286-92. Epub 2008 Jan 16. Cited in Pubmed; PMID 18199675. doi: 10.1529/biophysj.107.119099. PMCID: PMC2275686.

Velázquez P, Cameron TO, Kinjo Y, Nagarajan N, Kronenberg M, Dustin ML. Cutting edge: activation by innate cytokines or microbial antigens can cause arrest of natural killer T cell patrolling of liver sinusoids. J Immunol. 2008 Feb 15;180(4):2024-8. Cited in Pubmed; PMID 18250405.

Selected Books and Reviews

Fooksman DR, Vardhana S, Vasiliver-Shamis G, Liese J, Blair DA, Waite J, Sacristán C, Victora GD, Zanin-Zhorov A, Dustin ML. Functional anatomy of T cell activation and synapse formation. Annu Rev Immunol. 2010 Mar;28:79-105. Cited in Pubmed; PMID 19968559. doi: 10.1146/annurev-immunol-030409-101308. PMCID: PMC2885351.

Dustin ML, Starr T, Varma R, Thomas VK. Supported planar bilayers for study of the immunological synapse. In: Coligan JE, Bierer BE, Margulies DH, Shevach EM, Strober W, editors. Current Protocols in Immunology. Unit 18.13. New York: John Wiley and Sons; 2007.

Lebdeva T, Dustin ML, Sykulev Y. Target cell contributions to cytotoxic T cell sensitivity. In: Kropshofer H, Vogt AB, editors. Antigen Presenting Cells. Weinheim: Wiley-VCH; 2005. pp. 199-215.

Dustin ML. Beta2 integrins and their ligands in inflammation. In: Ley K, editor. Physiology of Inflammation. New York: Oxford University Press; 2001. pp. 242-62.

Dustin ML, Springer TA. Intercellular Adhesion Molecules (ICAMs) and LFA-1. In. In: Kreis T, Vale R, editors. Guidebook to the Extracellular Matrix, Anchor and Adhesion Proteins. Oxford: Oxford University Press; 1999. pp. 154-7, 216-20, 228-32.

Organizational Associations

American Society of Cell Biology; American Association of Immunologists

Selected Awards

1983  Phi Beta Kappa
1984  Sheilds Warren Award, Boston University
1986  Ryan Fellowship, Harvard University
1997  Englicheff Award, Arthritis Foundation
2000  Presidential Early Career Award in Science and Engineering
2000  Highly Cited Researcher in Immunology. Institute for Scientific Information
2001  Keynote Speaker, FASEB Autoimmunity Conference
2001  Irene Diamond Professorship in Immunology
2004  Special presentation, Workshop on CD1d and NKT cells, Heron Island Australia
2005  Keynote Speaker, University of Texas Immunology Retreat
2006  Keynote Speaker, "Imaging the immune synapse" Paris, France
2006  Keynote Speaker, "Zoo meeting on cellular interactions" Amsterdam
2006  Keynote Speaker, Harvard Medical School Immunology Program Retreat
2007  Keynote Speaker, Biology in Motion, Cell Press and MGH, Evian, France
2010 Invited Speaker at NIH's Wednesday Afternoon Lecture Series, "Creating Super-Regulatory T Lymphocytes"
2010 Muriel G. and George W. Singer Professor of Molecular Immunology
2010 Elected Fellow of the American Association for the Advancement of Science (AAAS)